109 23 89 KNOCK-IN MOUSE HOMOLOGOUS RECOMBINATED BY BANK VOLE'S PRION PROTEIN GENE WHICH 109TH AMINO ACID IS METHIONINE AND FROM 23TH TO 89TH AMINO ACID CODING SEQUENCES ARE ELIMINATED AND APPLICATIONS THEREOF

摘要

The present invention relates to a knock-in mouse homologous recombinated with a bank vole′s prion protein gene in which the 109th amino acid is methionine and a sequence encoding from the 23rd to the 89th amino acids is deleted; and an application of the same. More specifically, the present invention relates to a knock-in mouse whose inherent prion protein gene is knocked-in with a cellular prion protein gene of a bank vole in which the 109th amino acid is methionine and a sequence encoding from the 23rd to the 89th amino acids is deleted, by a homologous recombination method; and an application of the same. According to the present invention, a knock-in mouse may be produced whose inherent prion protein gene is replaced (substituted) with the gene of the prion protein of a bank vole in which the 109th amino acid is methionine and a sequence encoding from the 23rd to the 89th amino acids is deleted by a homologous recombination method, and the knock-in mouse thus produced may be used beneficially as an animal model used to evaluate the anti-prion validity of a candidate material of a drug developed to treat prion disease including CJD. Further, the present invention may be beneficially used in studies to identify a role in the emergence of prions or to improve the performance of an anti-prion material.