Molecularly imprinted nano-cavities capable of size, shape and ligand binding domain recognition for proteins bearing high homologous amino acid sequences and sizes

摘要

Proteins possess unique functions and play important roles in biological systems, depending on their differences in amino acid sequences, three-dimensional structures and specific ligand binding domains (LBDs). Vascular endothelial growth factor (VEGF)-A, an angiogenesis factor, has mainly three isoforms such as VEGF165, 189 and 121; VEGF 165 and 189 possess common ligand binding domains for heparins (HBD) with a highly homologous amino acid sequences (87 %), whereas no HBD is on VEGF121.