109 23 89 KNOCK-IN MOUSE HOMOLOGOUS RECOMBINATED BY BANK VOLE'S PRION PROTEIN GENE WHICH 109TH AMINO ACID IS METHIONINE AND FROM 23TH TO 89TH AMINO ACID CODING SEQUENCES ARE ELIMINATED AND APPLICATIONS THEREOF

摘要

The present invention relates to a dissolved mouse homologous recombination with the prion protein gene of the embankment mouse, in which the 109th amino acid is methionine and the sequences encoding the 23rd to 89th amino acids, and more specifically, the 109th amino acid is methionine ( Cellular prion protein gene of bank vole from which the genes encoding amino acids 23 and 89 were removed and replaced by the knock-in homologous recombination method. A banked ratified transgenic mouse and its application technology. According to the present invention, a homologous recombination method is performed for the embankment-developed mice in which the 109th amino acid is methionine and the prion protein gene of the embankment mouse is removed from the 23rd to 89th amino acid sequence. It can be usefully used as an animal model used to evaluate the anti-prion efficacy of therapeutic candidates developed for the purpose of treating prion diseases including CJD. In addition, it can be useful to find out the role of the prion generation process or to study the performance of the anti-prion material.