SRM / MRM assay for tubulin beta-3 chain (TUBB3) protein

摘要

The present invention relates to a specific peptide from tubulin beta-3 chain protein (TUBB3) which is particularly advantageous for quantitating TUBB3 protein directly in a biological sample immobilized on formalin by selective reaction monitoring (SRM) mass spectrometry and the induced ionization properties of said peptide . Protein samples are prepared from biological samples using liquid tissue reagents and protocols and TUBB3 protein is quantitated by SRM / MRM mass spectrometry of samples, where specific peptides are quantified. A method of treating a patient with a taxane-based therapeutic regimen is provided when the measured level of TUBB3 in a patient tumor sample is compared to a baseline level and the measured TUBB3 level is below a baseline level. A suitable reference level is, for example, about 700 amol / ug tissue.