The manufacturing method of mixed ligands bound silica monolith particles for separation of proteomic samples and chromatographic columns comprising the mixed ligands bound silica monolith particles manufactured by the method

摘要

The present invention relates to a producing method of mixed ligand-attached silica monolith powder for proteomic sample separation analysis, and a column for chromatography including mixed ligand-attached silica monolith powder produced by the same. The present invention sets a special strategy to remarkably improve performance and, more specifically, introduces a producing method of a mixed ligand-attached chromatographic stationary phase, which: uses silica monolith powder having excellent column charging efficiency as a base material; mixes a polar ligand having an element functional group at an end with a ligand of a non-polar hydrocarbon chain as a stationary ligand to exclude too strong interactions between stationary phase-analysis materials; and strictly controls the mixing ratio of the two ligands to prevent sticky characteristics between stationary phase particles. The present invention has an effect of dramatically improving separation efficiency compared to a conventionally reported mixed ligand stationary phase when using the mixed ligand-attached silica monolith powder of the present invention as a chromatography stationary phase.