Expression vector for secretory production of the target protein in Pseudomonas putida and method for producing the target protein using the same

摘要

The present invention relates to an expression vector for secretory production of a target protein, comprising a gene of the target protein and a gene encoding an N-domain region of FliC protein; a transformant transformed with the expression vector; and a method for producing a target protein using the transformant. The present invention has confirmed that the specific amino acid sequence of the N-domain region of the FliC protein is related to the secretion and secretion amount of the protein in the production of the target protein using the protein secretion mechanism of Pseudomonas putida strain, and thus can be advantageously utilized industrially by producing a variety of useful materials, including lignin-degrading enzymes through the Pseudomonas putida strain.