METHOD FOR PRODUCING A HETEROGENEOUS PREPARATION BASED ON BROMELEINE Possessing Wound-healing Properties

摘要

FIELD: pharmaceuticals.SUBSTANCE: invention relates to the chemical-pharmaceutical industry and is a process for the preparation of a heterogeneous preparation based on bromelain having wound-healing properties, including immobilization of the enzyme preparation in a buffer solution, incubation and washing, characterized in that immobilization of bromelain is carried out on a matrix of ion-exchange fibers VION KN-1 or VION AN-1 in a ratio of 20 ml of enzyme solution at a concentration of 2 mg/ml per 1 g of fibers; a 0.05 M glycine buffer with a pH of 9.0–10.5 or 0.05 M borate buffer with a pH of 8.0 without addition of KCl is used as the buffer solution for immobilization on the VION KN-1, and for immobilization on VION AN-1 – 0.2 M acetate buffer with pH 5.0; incubation is carried out for 24 hours at room temperature; the formed precipitate is washed with the buffer used in the immobilization until the protein in the washing waters is absent.EFFECT: invention makes it possible to obtain a wound healing material based on bromelain, which will reduce the expense of wound healing due to the high stability of the preparation at temperatures above physiological and its prolonged action.1 cl, 7 dwg, 2 ex