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METHOD AND DEVICE FOR ASSESSING THE NUMBER OF MICROORGANISMS IN A TAXONOMIC UNIT IN SAMPLE
METHOD AND DEVICE FOR ASSESSING THE NUMBER OF MICROORGANISMS IN A TAXONOMIC UNIT IN SAMPLE
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机译:评估样品中分类生物单位中微生物数量的方法和装置
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摘要
Methods and apparatus to identify and quantify the microorganisms present in a sample. Sequence reads are classified using existing methods, but the classification results are corrected to account for the number of reads expected to be falsely classified as determined through simulation. With statistics on the expected number of reads misclassified, a linear least squares method (non-negative or otherwise) or other related technique can be used to adjust the number of reads that are classified to various taxonomic units (e.g., species) and to determine more accurate values for the quantities of those taxonomic units actually present in the sample, eliminating microorganisms in taxonomic units falsely determined to be present in the sample.
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