METHOD FOR ASSESSING THE METABOLIC ACTIVITY OF THE BONE MARROW MEGAKARYOCYTE SPROUT

摘要

FIELD: medicine.;SUBSTANCE: invention relates to medicine and is a method for assessing the metabolic activity of a megakaryocytic sprout of the bone marrow, including counting the number of megakaryocytes in the bone marrow, characterized in that a bone marrow aspirate is examined in a volume of 0.3–0.5 ml, which is transferred to a tube with EDTA, centrifuged at 3,000 rpm for 5 minutes, 250–400 mcl of cell-free myeloplasma are taken and its biochemical study is performed to determine the activity of alanine aminotransferase and aspartate aminotransferase by methods recommended by IFCC; calculate the metabolic activity of the megakaryocyte germ (PMAM) according to the formula: PMAM=A*100/B+C, where A is the number of megakaryocytes in 1 mcl of the bone marrow; B – activity of alanine aminotransferase in myeloplasma, E/l; C – activity of aspartate aminotransferase in myeloplasma, E/l; PMAM10.9 indicates a decrease in the metabolic activity of the megakaryocytic sprout of the bone marrow; PMAM15.9 indicates a high metabolic activity of the megakaryocytic sprout of the bone marrow; PMAM from 10.9 to 15.9 indicates a normal metabolic activity of the megakaryocytic sprout of the bone marrow.;EFFECT: method for estimating the metabolic activity of a megakaryocytic bone marrow sprout is proposed.;1 cl, 2 tbl, 3 ex