PREPARATION OF TRITICUM AESTIVUM WHEAT PROTEINASE OBTAINED IN SOLUBLE FORM AND METHOD FOR PREPARATION THEREOF

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to the field of molecular biology and biotechnology and is a biologically active protein preparation, having specific activity of papain-like cysteine proteinases, characterized in that it is an amino acid sequence selected from SEQ ID NO:2-4, expressed in soluble form. Invention also relates to a method for producing a biologically active protein preparation, wherein the method comprises transforming cells with plasmids containing DNA, encoding a protein having an amino acid sequence selected from SEQ ID NO: 2-4, cultivation and isolation of a biologically active preparation.;EFFECT: invention provides the possibility of obtaining a Triticum aestivum preparation with a high and stable yield, a high level of purification and functional activity.;9 cl, 8 dwg, 7 ex