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METHOD OF PRODUCING MATERIAL WITH HIGH TITRE OF HEPATITIS E VIRUS AND ANALYSIS OF HEPATITIS E VIRUS TITRATION
METHOD OF PRODUCING MATERIAL WITH HIGH TITRE OF HEPATITIS E VIRUS AND ANALYSIS OF HEPATITIS E VIRUS TITRATION
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机译：高滴度肝炎病毒材料的制备方法及肝炎病毒滴定分析
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摘要
FIELD: medicine.;SUBSTANCE: group of inventions relates to a method for producing a high titre of hepatitis E virus (HEV), comprising a) culturing a cell line in vitro, where the cell line is HepG2 (HB-8065 in the American Type Culture Collection (ATCC)) or HepG2/C3A (CRL-10741 in ATCC), in a medium containing polybrene in concentration of 1 mcg/ml to 5 mcg/ml, and b) infecting said cell line with HEV virus. Group of inventions also relates to a method of determining presence and / or level of HEV in a sample; culture medium for producing high HEV titre containing polybrene in range of 1 mcg/ml to 5 mcg/ml; method of examining HEV titration, comprising using a cell line, which is HepG2 (number HB-8065 in ATCC) or HepG2/C3A (number CRL-10741 in ATCC), and polybrene in range of 1 mcg/ml to 5 mcg/ml.;EFFECT: group of inventions provides high HEV virus titre.;16 cl, 5 ex, 5 dwg, 2 tbl
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机译：发明领域本发明涉及产生高滴度的戊型肝炎病毒（HEV）的方法，该方法包括：a）在体外培养细胞系 ，其中所述细胞系为HepG2。 （美国典型培养物保藏中心（ATCC）中的HB-8065）或HepG2 / C3A（ATCC中的CRL-10741），在含有浓度为1 mcg / ml至5 mcg / ml的聚乙烯的培养基中，b）感染所述细胞符合戊型肝炎病毒。一组发明还涉及确定样品中HEV的存在和/或水平的方法。用于生产含有1mcg / ml至5mcg / ml范围的聚乙烯的高HEV滴度的培养基;检测HEV滴定的方法，包括使用细胞系，其为HepG2（ATCC中为HB-8065）或HepG2 / C3A（ATCC中为CRL-10741），并且聚乙烯的范围为1 mcg / ml至5 mcg / ml效果：一组发明提供高HEV病毒滴度; 16 cl，5 ex，5 dwg，2 tbl
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