METHOD FOR GENOME DETECTION OF CORONAVIRUS INFECTION CAUSATIVE AGENT IN CATTLE

摘要

FIELD: biotechnology.;SUBSTANCE: described is a method for detecting the coronavirus infection pathogen genome in cattle. Method includes isolation of RNA from biological material in form of extract of excrements or fragments of abdominal cavity by sorption method, synthesis of cDNA on RNA matrix by single-step staging with addition of internal and positive control samples of multiplex reverse transcription reaction and polymerase chain reaction - with 45 cycles of amplification with real-time detection using oligonucleotide primers specific for the virus genome region of fluorescent-labelled probe. Fragment of the coronavirus infection agent genome has the following nucleotide sequences: BCoVF 5'-GATCAAATTGCTAGT-3' - forward primer; BCoVR 5'-CAGTCTGCTTAGTTA-3' - reverse primer; BCoVP 5'-FAM-GGATGCCACTAAGCCA-3'-BHQ1 is a probe.;EFFECT: invention extends the range of methods for diagnosing coronavirus infection in cattle.;1 cl, 4 tbl, 1 ex