EFFICIENT METHOD FOR ESTABLISHING INDUCED PLURIPOTENT STEM CELLS

摘要

The present invention provides a method of improving the efficiency of establishment of induced pluripotent stem cell, including increasing, in a nuclear reprogramming step of somatic cell, the level of activated form of one or more proteins selected from the group consisting of Ras family members, PI3 kinase, RalGEF, Raf, AKT family members, Rheb, TCL1 and S6K. The present invention also provides an agent for improving the efficiency of establishment of induced pluripotent stem cell, containing a factor selected from the group consisting of Ras family members, PI3 kinase, RalGEF, Raf, AKT family members, Rheb, TCL1 and S6K, and nucleic acids encode the same. Furthermore, the present invention provides a method of producing induced pluripotent stem cells, including contacting a somatic cell with a nuclear reprogramming substance and one or more factors selected from the group consisting of Ras family members, PI3 kinase, RalGEF, Raf, AKT family members, Rheb, TCL1 and S6K, and nucleic acids that encode the same.