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MALIGNANT GLIOMA CAR-T THERAPEUTIC VECTOR BASED ON OCTS TECHNOLOGY, AND CONSTRUCTION METHOD AND APPLICATION THEREOF
MALIGNANT GLIOMA CAR-T THERAPEUTIC VECTOR BASED ON OCTS TECHNOLOGY, AND CONSTRUCTION METHOD AND APPLICATION THEREOF
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机译：基于OCTS技术的恶性胶质瘤CAR-T治疗载体及其构建方法与应用
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摘要
The invention discloses an OCTS-based CAR-T vector for treating spongioblastoma, consisting of lentiviral skeleton plasmid, human EF1α promoter (SEQ ID NO.14), OCTS chimeric receptor structural domain, and PDL1 single-chain antibody; the OCTS chimeric receptor structural domain consists of CD8 leader chimeric receptor signal peptide (SEQ ID NO.15), PDL1 single-chain antibody light chain VL (SEQ ID NO.16), PDL1 single-chain antibody heavy chain VH (SEQ ID NO.17), EGFRvIII single-chain antibody light chain VL (SEQ ID NO.18), EGFRvIII single-chain antibody heavy chain VH (SEQ ID NO.19), antibody Inner-Linker (SEQ ID NO.20), single-chain antibody Inter-Linker (SEQ ID NO.21), CD8 Hinge chimeric receptor linker (SEQ ID NO.22), CD8 Transmembrane chimeric receptor transmembrane domain (SEQ ID NO.23), TCR chimeric receptor T cell activation domain (SEQ ID N0.26) and chimeric receptor co-stimulator domain. Also, the invention discloses the preparation method and application in the preparation of drugs for treating spongioblastoma of the vector.
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机译：本发明公开了一种基于OCTS的治疗海绵状母细胞瘤的CAR-T载体，其由慢病毒骨架质粒，人EF1α启动子（SEQ ID NO.14），OCTS嵌合受体结构域和PDL1单链抗体组成。 OCTS嵌合受体结构域由CD8前导嵌合受体信号肽（SEQ ID NO.15），PDL1单链抗体轻链VL（SEQ ID NO.16），PDL1单链抗体重链VH（SEQ ID NO。 .17），EGFRvIII单链抗体轻链VL（SEQ ID NO.18），EGFRvIII单链抗体重链VH（SEQ ID NO.19），抗体内部接头（SEQ ID NO.20），链抗体Inter-Linker（SEQ ID NO.21），CD8铰链嵌合受体接头（SEQ ID NO.22），CD8跨膜嵌合受体跨膜结构域（SEQ ID NO.23），TCR嵌合受体T细胞活化结构域（SEQ ID NO。 N0.26）和嵌合受体共同刺激域。另外，本发明还公开了用于治疗所述载体的成纤维细胞瘤的药物的制备方法和应用。
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