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METHODS AND COMPOSITIONS FOR PREVENTING CONCATEMERIZATION DURING TEMPLATE- SWITCHING
METHODS AND COMPOSITIONS FOR PREVENTING CONCATEMERIZATION DURING TEMPLATE- SWITCHING
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机译:防止模板切换过程中高等级化的方法和组成
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摘要
Compositions and methods for performing a template-switching reaction are provided that may include reducing or eliminating concatemerization of the template-switching oligonucleotide (TSO). In some embodiments, the composition may comprise a site-specific nucleic acid cleaving enzyme, a TSO that includes a recognition sequence for a site-specific nucleic acid cleaving enzyme, wherein the TSO hasat its 3' end at least one nucleotide that is complementary to one or more non-templated nucleotides added to a templated cDNA strand by the reverse transcriptase, and a site-specific nucleic acid cleaving enzyme that cleaves the TSO at the recognition sequence.
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