BETA-GALACTOSIDASE FROM L. BULGARICUS FOR THE SYNTHESIS OF GALACTOOLIGOSACCHARIDES IN WHEY

摘要

The present invention relates to a process for the preparation of galactooligosaccharides (GOS) or a composition containing galactooligosaccharides, in which a beta-galactosidase (also lactase [EC3.2.1.23]) derived from L. bulgaricus (L. delbrueckii spp.bulgaricus) is obtained. at a temperature of 37 ° C or less with a lactose-containing composition such as milk, buffer or whey, e.g. Sweet whey, sour whey, whey concentrate or whey permeate, incubated, or a use of the enzyme for the production of galactooligosaccharides or a galactooligosaccharide-containing composition in such a lactose-containing composition. A method for producing a galactooligosaccharide-containing composition with a defined proportion of DP4 galactooligosaccharides and DP3 galactooligosaccharides in all galactooligosaccharides is also provided. The invention further provides compositions which can be prepared by the processes according to the invention and, in this case, beta-galactosidase prepared in casein peptone medium and processes for their production.