A method for isolating a T cell receptor that has antigen specificity for cancer-specific mutations

摘要

PROBLEM TO BE SOLVED: To provide a method for isolating a T cell receptor having antigen specificity for a cancer-specific mutation. A method of isolating a TCR having antigen specificity for a mutated amino acid sequence encoded by a cancer-specific mutation, wherein each gene encodes a mutated amino acid sequence. Identifying one or more genes in the nucleic acid of a patient's cancer cells that contain a mutated mutation; inducing the patient's auto-APC to present a mutated amino acid sequence; Co-culturing with an autologous APC that presents a mutated amino acid sequence; selecting an autologous T cell; and isolating a nucleotide sequence encoding the TCR from the selected autologous T cell, TCR A method having antigen specificity for a mutated amino acid sequence encoded by a cancer-specific mutation. [Selection diagram] None