Ion-permeable lipid bilayer membrane formation method and current measurement apparatus for ion-permeable lipid bilayer membrane formation

摘要

[Problem] To more accurately measure the ion channel current of an ion-permeable lipid bilayer membrane. [Solution] This method for forming an ion-permeable lipid bilayer membrane is provided with: a step S501 for placing, on an electrode base, a plate that has on the upper surface thereof at least a pair of wells capable of holding a liquid therein, the wells being disposed so that the pair of wells communicate to each other, and bringing at least a pair of electrodes communicating the interior of the wells and the bottom surface of the plate to each other and at least a pair of contacts provided to the electrode base into contact with each other; a step S502 for fixing the plate and the electrode base, using an urging means for urging the contacts towards the electrodes and a fixing means for fixing the plate and the electrode base, so that the electrodes and the contacts are in contact with each other; a step S503 for forming a lipid bilayer membrane; and a step S504 for applying a constant voltage between the pair of electrodes and measuring the current between the pair of electrodes using an ammeter.