Antibody-antigen mixture detection method using surface acoustic wave sensor and surface acoustic wave sensor

摘要

PROBLEM TO BE SOLVED: To detect whether or not a premixing reaction of a sample containing an antigen and a secondary antibody is sufficient when using a surface acoustic wave sensor and a sandwich assay method to detect the concentration of an antigen in a sample such as blood. To do. SOLUTION: A secondary antibody of a sandwich assay method and an antigen are introduced in separate introduction paths 11 and 12, merged in a merging section 13, and mixed in a discharge path 14 with a microchannel 1 and a secondary. A second detection region 221-n on which an antibody is immobilized and a second comb-shaped electrode 222-n that transmits and receives surface acoustic waves or transmits and reflects surface acoustic waves are provided, and the respective second detection regions 221-n are provided. However, the surface acoustic wave sensor S is provided with a plurality of second channels 22-n arranged inside the discharge path 14 and at different distances from the merging portion 13. [Selection diagram] Fig. 2