Bacillus subtilis mutant strain and its use

摘要

PROBLEM TO BE SOLVED: To provide a bacillus-subtilis mutant in which a large region of the genome is deleted with respect to the wild type strain and which can produce an interest-gene product at a high yield, and to provide methods of producing interest-genes using the bacillus-subtilis mutant.SOLUTION: A present invention provides a bacillus-subtilis mutant having a genome in which one or more regions selected from the group consisting of prophage 6 region, prophage 1 region, prophage 4 region, PBSX region, prophage 5 region, prophage 3 region, spb region, pks region, skin region, pps region, prophage 2 region, ydcL-ydeK-ydhU region, yisB-yitD region, yunA-yurT region, cgeE-ypmQ region, yeeK-yesX region, pdp-rocR region, ycxB-sipU region, prophage 7 region (yrkM-yraK, or yrkS-yraK), sbo-ywhH region, yybP-yyaJ region and yncM-fosB region, are deleted, wherein genes are constructed so that abrB gene or its corresponding gene is constantly expressed, and wherein kinA genes are deleted or inactivated.SELECTED DRAWING: None