Method of manufacturing polymer by native chemical ligation method

摘要

An object of the present invention is to provide a method for producing a polymer having a peptide bond in a high yield and in a short time. According to the present invention, it is a method for producing a polymer having a peptide bond, wherein (A) a polymer fragment 1 having an unprotected cysteine residue and a protected cysteine residue and being activated. A step of ligating the polymer fragment 2 having an arbitrary amino acid residue obtained by the NCL method to obtain a polymer fragment 1-2 having a protected cysteine residue, and (B) the step (A). Later, a deprotecting agent was added to obtain a polymer fragment 1-2 having an unprotected cysteine residue, and steps (A) and (B) were carried out by the deprotecting agent inactivating agent. A production method is provided that is carried out in an existing reaction system. Step (A), step (B) and step (C) can be repeated one or more times.