DETERMINATION METHOD FOR DETERMINING PRESENCE OF TWO OR MORE KINDS OF SPECIFIC OLIGONUCLEOTIDE, AND METHOD FOR DETERMINING THAT TEST OBJECT IS AFFECTED BY SMALL-CELL LUNG CANCER OR BILE DUCT CANCER OR HAS RISK OF BEING AFFECTED BY SUCH CANCER BY USING THE DETERMINATION METHOD

摘要

To provide a method for simply and accurately determining the presence of two or more kinds of specific oligonucleotide.SOLUTION: A determination method includes: a process of mixing a probe having a sequence complementary with two or more kinds of specific oligonucleotide and a measurement sample to obtain a mixture; a process of applying voltage between first solution containing the mixture and second solution which are partitioned by a lipid double membrane having a nanopore, and measuring the electric current intensity of electric current flowing between the first solution and the second solution over time to obtain electric current data over time; and a process of obtaining a plurality of interval time from the electric current data over time, performing a processing based on the central limit theorem regarding the plurality of interval time to obtain frequency distribution, identifying a content pattern derived from the two or more kinds of specific oligonucleotide on the basis of the frequency distribution, and determining the presence of each of the two or more kinds of specific oligonucleotide in the measurement sample from the content pattern.SELECTED DRAWING: Figure 1