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Recombinant Escherichia coli for high efficiency production of fructosylated chondroitin and method for making thereof

机译：用于高效生产果糖基软骨素的重组大肠杆菌及其制备方法

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摘要

The present invention relates to the field of biotechnology engineering. It provides a recombinant Escherichia coli that can produce fructosylated chondroitin with high yields and the method for constructing the recombinant strain. The present invention discloses a method for constructing an expression plasmid pETM6R1-RBS-glmM-GGGS-glmS that contains a glmM and a glmS gene under regulation of ribosome binding sites. The recombinant plasmid was transformed into E. coli K4 to obtain a recombinant E. coli strain ZQ33 that can produce fructosylated chondroitin up to 3.99 g·L^−1by fed-batch fermentation in a 5-L fermentor, which was increased by 108.90% compared to that of the wild type strain.

机译：本发明涉及生物技术工程领域。它提供了一种可高产率生产果糖基软骨素的重组大肠杆菌，以及该重组菌株的构建方法。本发明公开了在核糖体结合位点的调控下构建包含glmM和glmS基因的表达质粒pETM6R1-RBS-glmM-GGGS-glmS的方法。重组质粒被转化入E。大肠杆菌 K4以获得重组 E。通过在5升发酵罐中分批补料发酵可生产高达3.99 g·L ^{＆＃x2212; 1 的果糖化软骨素的大肠杆菌菌株ZQ33，与之相比增加了108.90％野生型菌株。} 展开▼

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公开/公告号US10508279B2

专利类型

公开/公告日2019-12-17

原文格式PDF

申请/专利权人 JING WU;QUAN ZHANG;LIMING LIU;XIULAI CHEN;JIA LIU;QIULING LUO;
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申请/专利号US201815891564

发明设计人 JING WU;QUAN ZHANG;LIMING LIU;XIULAI CHEN;JIA LIU;QIULING LUO;
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申请日2018-02-08

分类号C12N1/20;C12N15/70;C12N9/10;C12N9/90;C12P19/24;C12P19/26;

国家 US

入库时间 2022-08-21 11:29:35

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1. 一种高效生产果糖软骨素的重组大肠杆菌及其构建方法 [P] . 中国专利： CN107312738B . 2020.03.06

2. 一种高效生产果糖软骨素的重组大肠杆菌及其构建方法 [P] . 中国专利： CN107312738A . 2017-11-03

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机译：用于高效生产果糖基软骨素的重组大肠杆菌及其制备方法

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