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Fine mapping and validation of QTL underlying fiber content and identification of SNP markers for marker assisted selection

机译:QTL潜在纤维含量的精细定位和验证,以及用于标记辅助选择的SNP标记的鉴定

摘要

A method for identifying a quantitative trait locus associated with desirable nutritional traits in canola includes: analyzing a population of canola plants or germplasm for desirable nutritional traits; determining the genotype of the canola plants or germplasm using at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111; mapping the canola plants or germplasm for the presence of a quantitative trait locus (QTL) associated with the markers; and associating the QTL with the desirable nutritional trait. An isolated and/or recombinant nucleic acid includes a sequence associated with a quantitative trait locus (QTL), wherein the QTL is associated with a desirable nutritional trait in a canola plant or germplasm and wherein the QTL is further associated with at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111.
机译:一种用于确定与低芥酸菜子中所需的营养性状相关的定量性状基因座的方法,该方法包括:分析低芥酸菜子植物或种质的种群以获得所需的营养性状;使用选自由SEQ ID NO:1至SEQ ID NO:111组成的组中的至少一种标记来确定油菜植物或种质的基因型;对低芥酸菜子植物或种质作图,以确定是否存在与标记相关的数量性状位点(QTL);并将QTL与理想的营养性状相关联。分离的和/或重组的核酸包括与定量性状基因座(QTL)相关的序列,其中所述QTL与低芥酸菜子植物或种质中所需的营养性状相关,并且其中所述QTL还与至少一种选择的标记相关联。选自SEQ ID NO:1至SEQ ID NO:111。

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