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SYSTEMS AND METHODS FOR AUTOMATED CELL SEGMENTATION AND LABELING IN IMMUNOFLUORESCENCE MICROSCOPY
SYSTEMS AND METHODS FOR AUTOMATED CELL SEGMENTATION AND LABELING IN IMMUNOFLUORESCENCE MICROSCOPY
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机译:免疫荧光显微镜中细胞自动分离和标记的系统和方法
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摘要
Various techniques are provided for performing automated full-cell segmentation and labeling in immunofluorescent microscopy. These techniques perform membrane segmentation and nuclear seed detection separate and independently from each other, then combine their results to identify cell boundaries. Some embodiments use texture- and kernel-based image processing to perform the method. In some embodiments, the method for obtaining membrane features disclosed herein can be used in conjunction with or separate from the nuclear features. The results can be used for a variety of purposes, including whole-area cell segmentation in fluorescence-based tissue imaging.
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