DEVICE FOR DERIVING ANTARCTIC CHIONODRACO RASTROSPINOSUS SKIN ANTIFREEZE PROTEIN

摘要

A device for manufacturing Antarctic chionodraco rastrospinosus skin antifreeze protein includes a de-frozen and cleaning device for de-frosting frozen grass carp skin and then cleaning carp skin; a skin slurry for making de-fat skin slurry; upper fat on the skin slurry being removed to get de-fat skin slurry; an ultrasonic generator for ultrasonic treatment to change organizational structure of skin antifreeze proteins of the slurry; a composite protease adder for adding composite protease added to the slurry; a collector receiving the slurry from the adder, and then the slurry are retained a time period and cooled to room temperature; then a 4000 g centrifugation is performed through 10 min; and a two-step filter connected to the collector for receiving the supernatant from the collector for performing a two-step ultra-filtration process so as to get skin antifreeze protein with molecular weight of 8000˜10000 Dalton and.