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SITE-SPECIFIC GENERATION OF PHOSPHORYLATED TYROSINES IN PROTEINS
SITE-SPECIFIC GENERATION OF PHOSPHORYLATED TYROSINES IN PROTEINS
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机译:蛋白质中磷酸化酪氨酸的特定位点生成
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摘要
Provided herein are novel materials and methods for site-specific incorporation of phosphotyrosines into proteins. The novel methods of the invention encompass the use of a novel aminoacyl tRNA synthetase capable of charging compatible tRNAs with a phosphotyrosine precursor. The phosphotyrosine precursor is then incorporated, site-specifically, into a protein at sites where phosphotyrosine residues are desired. The phosphotyrosine precursors are subsequently treated to convert them into phosphotyrosine residues, yielding proteins with phosphotyrosines at selected sites. The scope of the invention encompasses novel aminoacyl tRNA synthetases, novel phosphotyrosine precursors, and methods of using these materials to create site-specific phosphorylated tyrosine residues in a protein.
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