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METHODS OF REDUCING DENSITY-DEPENDENT GC BIAS IN AMPLIFICATION
METHODS OF REDUCING DENSITY-DEPENDENT GC BIAS IN AMPLIFICATION
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机译:降低放大倍数相关的GC偏差的方法
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摘要
The invention provides methods for amplifying nucleic acids, particularly methods for reducing density-dependent GC bias and for reducing nucleic acid damage in a bridge amplification of a nucleic acid template. The invention also provides methods for evaluating the effect of reagents and/or additives on nucleic acid damage during bridge amplification of nucleic acid template strands. The methods are suited to solid phase amplification, for example, utilizing flow cells.
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