method for determining the presence or concentration of at least one analyte in a sample, method for determining the presence or concentration of antigen in a sample, method for determining the presence or concentration of a target nucleotide sequence in a sample and digital assay system to determine an analyte concentration in a sample

摘要

these are systems, devices and methods for the fast and accurate measurement of analytes by testing binding events, by direct and digital measurement of individually resolved reporter / analyte binding events. the digital molecular test systems, devices and methods disclosed in this document are capable of reading particle by particle using optical reporter molecules that detect and report the binding of a single analyte molecule and report each binding in binary format. such systems, devices and digital molecular test methods are useful in a variety of applications, such as mobile electronic devices for use in the field.