Application of the method of partial least squares to determine chromophore concentrations from fluorescence spectra of turbid samples

摘要

Abstract: An non-invasive optical method for obtainingquantitative biochemical information is desirable.While quantitative methods for predicting fluorophoreconcentrations in dilute solutions are well known,human tissue is highly scattering and absorbing andcannot be accurately treated with these techniques. Wehave employed the method of partial least squares todevelop an empirical, linear model of samplefluorescence from a training set with opticalproperties and known concentrations representative ofthose to be predicted. This model can be applied topredict chromophore concentrations in the unknownsamples.!9