recombinant expression of pcv2b orf2 protein in insect cells

摘要

the present invention relates to the field of veterinary vaccines, in particular porcine vaccines against pcv2 and associated diseases. specifically, the invention relates to the discovery that a mutation in the pcv2b orf2 protein is required to prevent its nuclear accumulation after expression in insect cells; the mutation introduces a proline at the amino acid position 131. this allows efficient expression in insect cells, easy collection and generates large amounts of virus-like particles. vlps are highly effective in vaccines for pigs to reduce pcv2 infection or associated signs of disease.