Recombinant expression of PCV2B ORF2 protein in insect cells

摘要

The present invention relates to the field of veterinary vaccines, in particular porcine vaccines against PCV2 and related diseases. Specifically, the present invention is required in the PCV2b ORF2 protein to prevent its nuclear accumulation when a mutation is expressed in an insect cell; It relates to the discovery that the mutation introduces proline at amino acid position 131. This allows efficient expression in insect cells, easy harvesting, and produces large amounts of virus-like particles. VLPs are very effective in porcine vaccines for the reduction of signs of infection or related disease by PCV2.