The present invention provides a strain of Bacillus subtilis selected from the group consisting of a) a strain deposited as DSM32324, b) a strain deposited as DSM32325, and c) a mutant strain according to (a) or (b) that is sensitive to ampicillin, vancomycin , gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol and having inhibitory activity against E. coli and Clostridium perfringens. The invention further relates to Bacillus compositions comprising at least one Bacillus subtilis strain of the invention, preferably a Bacillus subtilis strain DSM32324 and / or a Bacillus subtilis strain DSM32325, as a probiotic (DFM), premix, animal feed supplement or animal feed . The invention provides a method for improving one or more indicators of animal productivity, selected from the group consisting of 1) increased weight gain (WG), 2) reduced coefficient of feeding (FCR), 3) lowered score for lesions from necrotic enteritis, 4) lowered frequency necrotic enteritis, 5) reduced mortality from necrotic enteritis, 6) increased European Production Effectiveness Factor (EPEF), and 7) reduced mortality, by feeding the animal the strain or composition of the present invention.
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