New derivatives of coumarin, method for producing them and application in the method of detection and/or determination of catalytic activity and stereoselectivity of the hydrolase reactions

摘要

the subject of the invention are derivatives of coumarin of general formula 5, where r3 and r4 are independently hydrogen, alkyl of c1 - c25, possibly a position? , beta,? group - oh, alkoksylowu0105 c1 - c5, nh2, cooh; alken c2 - c25, alkin c2 - c6 - c10 or aryl c25; r5, r6, r7, r8, r9 are independently hydrogen, alkyl of c1 - c5, alkoksyl c1 - c5, phenyl, - cl, - br - j no2, triflorometyl, trichlorometyl,atom and x is oxygen, sulphur or nitrogen, their manufacture and their use as substrates for fluorescent colour and enzyme activity via spektrofotometrycznych, indications and to determine the stereoselektywnou015bci katalizowanych reaction for indications biochemicz this chemical, biotechnological, enzymologicznych.