Method of producing medical dressing using pig skin and medical application thereof

摘要

The purpose of the notification is to obtain a pig skin dressing comprising the collection and preparation of the material by cleaning the material in buffered washing liquids, preservation in a preservative solution, placing in a sealed package, washing and washing, chilling, lyophilisation,sterilisation. This is characterized by removing the outer layer of the skin from the collected material and then the material is cleaned in buffered washing liquids and rolled, then washed in an ultrasonic bath, keeps under vacuum closure in a solution containing DMSO and glycerol in quantities of v 1-15% DMSO and glycerol 85-99% used in a quantity of 2-10 ml per 1-10 cm2 sample for a period of time from one minute to ten minutes at the rate 1-C-20-C, after which the material is cooled by 10-30 min at the temperature from 1-C to -20-C, and then undergoes a gradual freezing first at temperatures from -10,C to -25,C,favourable -20-C for four to eight hours, then at a temperature from -50-C to -85-C favourable -75-C for 30-60 hours, after which the whole is incubated in successive 30-60 hours in liquid nitrogen pairs and 30-60 hours in liquid nitrogen, The material is then defrosted at room temperature for 1-8 hours in buffered washing liquids and washes with simultaneous rolling, then washed in an ultrasonic bath, lyophilised, sealed in a sealed container in an inert gas atmosphere and then sterilised.