Polyclonal IgY antibodies specific for Zika virus NS2B-NS3 protease, method of their production and their use

摘要

IgY-class polyclonal antibodies specific to Zika NS2B-NS3 protease isolated from egg yolks of poultry immunised with antigen in the form of recombinant NS2B-NS3 protease are reported. The mode of production of IgY-class polyclonal antibodies specific to Zika NS2B-NS3 protease is characterised by the immunisation of the antigen poultry in the form of NS2B-NS3 protein of the sequence shown in model 2, with immunisation being carried out at four separate doses, And as an adjuvant, you use the full Freund adjuvant,then isolate antibodies with 80-150 mg/egg efficiency and 85-95% purity by a known method. The notification also covers the use of IgY-class polyclonal antibodies specific to Zika NS2B-NS3 protease for in vitro diagnosis of Zika virus-induced diseases.