PERSONALIZED 3D NEURAL CULTURE SYSTEM FOR GENERATING HUMAN OLIGODENDROCYTES AND STUDYING MYELINATION IN VITRO

摘要

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 18 October 2018 (18.10.2018) W I PO I PCT IiiimmoimiolollmonolomomimominumovoimIE (10) International Publication Number WO 2018/191656 Al (51) International Patent Classification: A61K 35/30 (2015.01) A61P 25/00 (2006.01) A61K 35/545 (2015.01) Cl 2N 5/00 (2006.01) (21) International Application Number: PCT/US2018/027552 (22) International Filing Date: 13 April 2018 (13.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/485,251 13 April 2017 (13.04.2017) US (71) Applicant: THE BOARD OF TRUSTEES OF THE LE- LAND STANFORD JUNIOR UNIVERSITY [US/US]; Office of the General Counsel, Building 170, 3rd Floor, Main Quad, P.O. Box 20386, Stanford, CA 94305-2038 (US). (72) Inventors: MARTON, Rebecca; 233 Ayrshire Farm Lane, Apt. 205, Stanford, CA 94305 (US). PASCA, Sergiu, P.; 201 Charles Marx Way, Palo Alto, CA 94304 (US). (74) Agent: SHERWOOD, Pamela, J.; Bozicevic, Field & Francis LLP, 201 Redwood Shores Parkway, Suite 200, Redwood City, CA 94065 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) (54) Title: PERSONALIZED 3D NEURAL CULTURE SYSTEM FOR GENERATING HUMAN OLIGODENDROCYTES AND = STUDYING MYELINATION IN VITRO (57) : Human pluripotent stem cells are differentiated in vitro in- to oligodendro-spheroids comprising oligodendrocytes for use in analysis, screening programs, and the like. Olig2 Nkx2.2 Hoechst W O 20 18/ 19 165 6 Al HG. 1A