Microbial enzymes for collagen-based tissue alpha-galactose reduction

摘要

An in vitro procedure for preparing a non-human tissue matrix for transplantation comprising: contacting a collagen containing tissue matrix with an isolated Trichoderma reesei or Clostridium cellulyticum alpha-galactosidase in an amount and 5 for a time sufficient to remove an α-gal epitope from the tissue matrix, thereby preparing the tissue matrix for transplantation in a human patient.