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Hydroxylase at position 4 of pipecolinic acid and method for producing 4-hydroxy amino acid using the same
Hydroxylase at position 4 of pipecolinic acid and method for producing 4-hydroxy amino acid using the same
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机译:胡椒酸第4位的羟化酶和使用该酶生产4-羟基氨基酸的方法
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摘要
The present invention provides a pipecolic acid 4-hydroxylase protein exemplified by the following (A), (B), and (C), having activity to react with L-pipecolic acid in the presence of 2-oxoglutaric acid and iron(II) ions to produce trans -4-hydroxy-L-pipecolic acid, and a method for producing 4-hydroxy amino acid, which method comprises reacting the pipecolic acid 4-hydroxylase protein, cells containing the protein, a treated product of the cells, and/or a culture liquid obtained by culturing the cells, with ±-amino acid to produce 4-hydroxy amino acid: (A) a polypeptide comprising the amino acid sequence represented by SEQ ID NO:2, 4, 6, 8, 10, 12, 16, or 18; (B) a polypeptide comprising the amino acid sequence represented by SEQ ID NO:2, 4, 6, 8, 10, 12, 16, or 18 except that one or several amino acids are deleted, substituted, and/or added, and having pipecolic acid 4-hydroxylase activity; and (C) a polypeptide having an amino acid sequence that is not less than 80% identical to the amino acid sequence represented by SEQ ID NO:2, 4, 6, 8, 10, 12, 16, or 18, and having pipecolic acid 4-hydroxylase activity.
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机译:本发明提供了以下述(A),(B)和(C)为例的胡椒酸4-羟化酶蛋白,其在2-氧戊二酸和铁(II)的存在下具有与L-胡椒酸反应的活性。离子产生反式-4-羟基-L-哌酸,以及产生4-羟基氨基酸的方法,该方法包括使哌酸4-羟化酶蛋白,含有该蛋白的细胞,该细胞的处理产物和/或通过用±氨基酸培养细胞以产生4-羟基氨基酸而获得的培养液:(A)包含由SEQ ID NO:2、4、6、8、10、12、16或18表示的氨基酸序列的多肽;(B)多肽,其包含由SEQ ID NO:2、4、6、8、10、12、16或18表示的氨基酸序列,除了一个或几个氨基酸被缺失,取代和/或添加,和具有胡椒酸4-羟化酶活性;和(C)具有与SEQ ID NO:2、4、6、8、10、12、16或18表示的氨基酸序列不小于80%同一性的氨基酸序列且具有哌考酸的多肽4-羟化酶活性。
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