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Pipecolinic acid 4-hydroxylase and method for producing 4-hydroxy amino acid using same
Pipecolinic acid 4-hydroxylase and method for producing 4-hydroxy amino acid using same
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机译:哌啶甲酸4-羟化酶及其使用相同的4-羟基氨基酸的方法
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摘要
The present invention provides a pipecolic acid 4-hydroxylase protein exemplified by the following (A), (B), and (C), having activity to react with L-pipecolic acid in the presence of 2-oxoglutaric acid and iron(II) ions to produce trans-4-hydroxy-L-pipecolic acid, and a method for producing 4-hydroxy amino acid, which method comprises reacting the pipecolic acid 4-hydroxylase protein, cells containing the protein, a treated product of the cells, and/or a culture liquid obtained by culturing the cells, with α-amino acid to produce 4-hydroxy amino acid:(A) a polypeptide comprising the amino acid sequence represented by SEQ ID NO:2, 4, 6, 8, 10, 12, 16, or 18;(B) a polypeptide comprising the amino acid sequence represented by SEQ ID NO:2, 4, 6, 8, 10, 12, 16, or 18 except that one or several amino acids are deleted, substituted, and/or added, and having pipecolic acid 4-hydroxylase activity; and(C) a polypeptide having an amino acid sequence that is not less than 80% identical to the amino acid sequence represented by SEQ ID NO:2, 4, 6, 8, 10, 12, 16, or 18, and having pipecolic acid 4-hydroxylase activity.
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机译:本发明提供一种百乙酸4-羟化酶蛋白,其举例如下(a),(b)和(c),在2-氧杂露酸和铁(II)存在下,具有与L-苯乙烯酸反应的活性产生反式-4-羟基-1-哌啶醇的离子,以及制备4-羟基氨基酸的方法,该方法包括使哌啶酸4-羟化酶蛋白,含有蛋白质的细胞,细胞的处理产物反应/或通过培养细胞获得的培养液,用α-氨基酸制备4-羟基氨基酸:(a)一种多肽,其包含由SEQ ID NO:2,4,6,8,10表示的氨基酸序列, 12,16或18;(b)除了缺失一个或几个氨基酸之外,包含由SEQ ID NO:2,4,6,8,10,12,16或18表示的氨基酸序列,除了缺失一种或几个氨基酸,取代和/或加入,并具有苯乙烯酸4-羟化酶活性; (c)具有氨基酸序列的多肽,其不小于80%与由SEQ ID NO:2,4,6,8,10,12,16或18表示的氨基酸序列相同,并且具有哌啶醇酸4-羟化酶活性。
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