Procedure for the production of an enzymatic cocktail from mushroom must

摘要

The invention relates to a method for producing an enzymatic cocktail from a cellulose-producing and/or hemicellulose-producing cellulolytic microorganism, comprising: - a step of producing enzymes with the obtaining of a medium containing enzymes and a microorganism must, said must being optionally separated from the liquid containing said enzymes, - a step of cooling said must to a temperature below the temperature of the enzyme-producing step, for a period of time such that the beta-glucosidase concentration of the liquid resulting from the cooling step is greater than that of the liquid resulting from the enzyme-producing step, and/or the ratio of the volume of solid/total volume is less than said ratio for the enzyme-producing step; and an enzymatic cocktail is obtained at the end of the cooling step.