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Procedure for the production of an enzymatic cocktail using solid residues from a biochemical conversion process of lignocellulosic materials
Procedure for the production of an enzymatic cocktail using solid residues from a biochemical conversion process of lignocellulosic materials
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机译:使用木质纤维素材料生物化学转化过程中的固体残留物生产酶促混合物的程序
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摘要
The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases: a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L; a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.
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