GRNA TARGETING SEQUENCE OF SPECIFIC TARGET HUMAN CD272 GENE AND APPLICATION THEREOF

摘要

A gRNA targeting sequence of a specific target human CD272 gene and an application thereof, relating to the technical field of gene editing. The gRNA targeting sequence has a nucleotide sequence of 5'-TGTTCCAGATGTCCAGATAT-3'. According to the gRNA targeting sequence, two single-stranded oligo sequences are designed and synthesized and then are annealed to form a double chain, and then the double chain is connected to a Cas9 vector; by using the Cas9 vector, gRNA and a CRISPR system are introduced into target cells, a Cas9 protein may find out a DNA sequence matched with the Cas9 protein under the guidance of the gRNA, shearing is performed, and the CD272 gene is knocked out.