Provided is a double-chain DNA biomarker for early diagnosis and preoperative evaluation of pheochromocytomas/paragangliomas. The biomarker is a double-chain DNA fragment specifically derived from the genome of a serum exosome of pheochromocytomas/paragangliomas. Also provided is a kit for detecting hepatitis B gene in pheochromocytomas/paragangliomas, which comprises a detection reagent for detecting the biomarker.
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