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METHOD FOR FULL COURSE DETECTION OF C-REACTIVE PROTEIN AND CORRESPONDING KIT

机译:C-反应蛋白和相应试剂盒的全过程检测方法

摘要

Provided in the present invention are a kit for performing full course detection of C-reactive protein on the basis of chemiluminescence immunoassay and a method for performing full course detection of the C-reactive protein. The kit comprises an R1 reagent, an M reagent, an R2 reagent, a pre-excitation solution, and an excitation solution. The R1 reagent, i.e. a sample treatment solution, is a 0.5M citric acid solution (pH 3.0-3.5, which is adjusted using disodium hydrogen phosphate dodecahydrate). Also provided in the present invention is a kit for performing full course detection of C-reactive protein, comprising a flat-bottomed chemiluminescence plate coated by a first antibody, the sample treatment solution, a horseradish peroxidase (HRP) or alkaline phosphatase (AP) labeled secondary antibody, and a color developing solution. Also provided in the present invention is a method for use in performing full course detection of C-reactive protein using the foregoing kit. Both the first antibody and the second antibody are monoclonal antibodies that may specifically react with the C-reactive protein.
机译:本发明提供了基于化学发光免疫测定法进行C反应蛋白的全过程检测的试剂盒和进行C反应蛋白的全过程检测的方法。该试剂盒包括R1试剂,M试剂,R2试剂,预激发溶液和激发溶液。 R1试剂,即样品处理溶液,是0.5M柠檬酸溶液(pH 3.0-3.5,可使用十二水合磷酸氢二钠调节)。在本发明中还提供了用于进行C反应蛋白的全过程检测的试剂盒,其包括由第一抗体,样品处理溶液,辣根过氧化物酶(HRP)或碱性磷酸酶(AP)包被的平底化学发光板。标记的二抗和显色溶液。本发明还提供了一种使用前述试剂盒进行C反应蛋白的全过程检测的方法。第一抗体和第二抗体都是可以与C反应蛋白特异性反应的单克隆抗体。

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