METHOD FOR PRODUCING CELLULASE AGENT, AND METHOD FOR PRODUCING SACCHARIFICATION/FERMENTATION PRODUCT IN WHICH SAID CELLULASE AGENT IS USED

摘要

Disclosed is a method for producing a cellulase agent. This method includes a step for culturing microorganisms of the genus Trichoderma at a pH of at least 1 and less than 5 in a medium containing a polysaccharide that has at least two β-1,4-bonded glucoses to produce a cellulase agent, the ratio of the β-glucosidase activity in the cellulase agent to the filter paper decomposition activity of the cellulase agent being lower than 3. Also disclosed are a method for producing a saccharification/fermentation product and a method for culturing surface layer presentation microorganisms using a culture medium that contains a cellulose material treated by a cellulase agent produced by the above method. These methods make it possible to produce a saccharification/fermentation product efficiently from a cellulose-based biomass irrespective of the location environment and production scale of a manufacturer.