AN IMPROVED METHOD TO PRODUCE CHEMICAL COMPOUNDS DERIVED FROM OXALOACETATE BY MICROORGANISMS

摘要

A method for the production of a chemical compound derived from oxaloacetate using genetically modified microorganismsis disclosed. The chemical compound can for example be ectoine or the ectoine precursor NADA. The genetic modifications increase the compound production by increasing the extracellular accumulation, decreasing the intracellular degradation, and by disrupting the PEP-pyruvate-oxaloacetate node of the tricarboxylic acid(TCA) cycle. The microorganisms obtained by the genetic modifications are disclosed as well, as well as their use in the production method. The microorganisms are preferably gram negative bacteria, such as halophilic bacteria, for example Halomonas elongata.