BIOFLUID SENSING DEVICES WITH INTEGRATED CHARGE SEPARATION BUFFERING

摘要

Devices and methods are described herein for separating electrically charged particles in a biofluid sensing device employing electrochemical aptamer-based ("EAB") sensors to limit ion-induced variability in the sensor outputs. In the disclosed embodiments, charge separation is accomplished with continuous biofluid sample flow in a sensor channel in order to limit ion exposure without decreasing the flow rate of biofluid through the device or increasing the biofluid volume required for sampling. Certain embodiments create an electric field to deflect ions away from sensors. Other embodiments create a magnetic field to accomplish charge separation. Y et other embodiments use a charged polymer membrane to electrostatically draw certain ions away from sensors. A method for reducing EAB sensor variability due to ions is also disclosed that uses such charge separation techniques within a biofluid channel.