首页>
外国专利>
High efficiency targeted in situ genome-wide profiling
High efficiency targeted in situ genome-wide profiling
展开▼
机译:高效靶向原位全基因组分析
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method for detecting the binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in a cell, including: contacting a permeabilized cell or nucleus with a specific binding agent that specifically recognizes the chromatin-associated factor of interest, wherein the specific binding agent is linked to a nuclease that is inactive or an activatable transposome; activating the nuclease or transposase, thereby excising the sequence of chromatin DNA bound to the chromatin- associated factor of interest; isolating the excised DNA; and determining the sequence of the excised DNA, thereby detecting binding of a chromatin-associated factor of interest to a sequence of chromatin DNA in the cell.
展开▼