DETECTION METHOD FOR MOLD CONTAMINATION IN TRADITIONAL CHINESE MEDICINAL MATERIAL

摘要

#$%^&*AU2020101360A420200820.pdf#####ABSTRACT The present invention provides a detection method for mold contamination of a traditional Chinese medicinal (TCM) material including the following steps: step 1. preparation of a mold contamination marker: performing extraction of a mold contaminated TCM material or a mold grown on a TCM material, filtration and solvent vaporization, adding a solvent for dissolution, loading into a liquid chromatography, collecting target peak fractions, and concentrating to obtain a mold contamination marker; step 2. preparation of a test sample solution: adding a solvent to a TCM material or a powdered TCM preparation sample, performing extraction, filtration and filtrate vaporization, adding a solvent to a residue for dissolution to obtain a test sample solution; step 3. analysis of a sample to be tested for mold contamination: determining that a sample has mold contamination if a chromatogram of a test sample shows a fluorescent spot having a color the same as that of the mold contamination marker or a peak at a position corresponding to the mold contamination marker. The present invention is effective in characterizing the quality of a TCM material (not including fungus medicinal material) or a powdered TCM preparation, thus avoiding use of mold contaminated TCM materials and improving safe clinical use of TCM materials. The method of the present invention is advantageous as it is convenient, sensitive and cost-effective.11/11 DADI Sig=3654Ref off(D Data-IelinIhtAnnmRhach201-10-23 Mold extract fromiHelhanthiAnrmRhachi012D mA 0.4 0).3 0.20.1 0 10 20 30 40 50 m DAD B Sig='2 54 ,4 Ref-off (D:DataoHeliantlu Annui Rhab 201810-23 Mold extract from %Helianthi Ani Rhach s 012.D) mAU: 2.5 2 1.5 1I 0.5 10 _ _ _ _ _ _ _ _ _ _ 2 0 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 4 0 _ _ _ _ _ _ _ _ _5 0 _ _ rn Figure 11a DAD I A. Sig=3654 Refoff (:DataoHeliathi Anm RhachQ2018S- 10-23 Mold extract from HehanthiAnnmu Rhachs 011 .D) mAU 120 100 80 60 40 20 20 40 60 80 100 mir DAD IB. S g-254,A Re eff (DDaoHehanth Annn Rhacho'2018 -10-23 2Mod extct from Heanthi Annm Rhachu 0 1 .D) mAU 500 400300 200 100 - ~l'J: ~ a 20 40 60 80 100 mi Figure 11b